Difference between revisions of "Part:BBa K1554006"
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<partinfo>BBa_K1554006 short</partinfo> | <partinfo>BBa_K1554006 short</partinfo> | ||
| − | . | + | The omega undercover vector has a BioBrick backbone but is compatible with GoldenBraid 2.0, a Golden Gate-based assembly system. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The omega undercovered rises as a way of trying to make BioBricks compatible with other Golden Gate-based assembly systems. It contains two BsmBI restiction sites which after digestion leave the overhangs required to perform a ligation of two transcriptional units which follow the required grammar. This grammar consists of the sequence GGAG on the 5' end and CGCT on the 3' end. Our team successfully used this vector in order to send [Part:BBa_K1554004](https://parts.igem.org/Part:BBa_K1554004) and [Part:BBa_K1554005](https://parts.igem.org/Part:BBa_K1554005) to the Registry of Parts. The molecular process involved in such ligation is explained in the following figure. | ||
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| + | https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg | ||
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Revision as of 18:09, 15 October 2014
Omega undercover
The omega undercover vector has a BioBrick backbone but is compatible with GoldenBraid 2.0, a Golden Gate-based assembly system. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]