Difference between revisions of "Part:BBa K1342001"
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ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion.
 
ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion.
 
We assembled ZinTp([https://parts.igem.org/Part:BBa_K1342005 BBa_K1342005]), RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), ApeA([https://parts.igem.org/Part:BBa_K1342002 BBa_K1342002]), Dter([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
 
We assembled ZinTp([https://parts.igem.org/Part:BBa_K1342005 BBa_K1342005]), RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), ApeA([https://parts.igem.org/Part:BBa_K1342002 BBa_K1342002]), Dter([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
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Revision as of 16:27, 15 October 2014

zinTp_AspA


Asp Device image.jpg


ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. We assembled ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002), Dter(BBa_B0015).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 600
    Illegal AgeI site found at 1452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1111


TLC Assay

Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)