Difference between revisions of "Part:BBa K1350006:Design"
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===Source=== | ===Source=== | ||
| − | + | From the genome of the BL21 | |
===References=== | ===References=== | ||
Latest revision as of 15:45, 15 October 2014
promoter+RBS(BBa_K608002)+Mdfa
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 552
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of Mdfa was from GenBank. After primer design, PCR and gene sequencing, we linked Mdfa to a strong promoter and RBS(BBa_K608002). Then the product, Mdfa device(BBa_K1350006) has been done.
Source
From the genome of the BL21