Difference between revisions of "Part:BBa K1316006"
Line 2: Line 2:
 
<partinfo>BBa_K1316006 short</partinfo>
 
<partinfo>BBa_K1316006 short</partinfo>
  
−
nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter
+
''nfsA'', ''nfsB'' and ''nemA'' (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter
  
 +
 +
<h3>Characterisation</h3>
  
 
The advantage of adding the N-genes has not been proven because the final constructs containing them (BBa_K1316007 and BBa_K1316008)  did not seem to be properly induced by the presence of 2,4-DNT.
 
The advantage of adding the N-genes has not been proven because the final constructs containing them (BBa_K1316007 and BBa_K1316008)  did not seem to be properly induced by the presence of 2,4-DNT.

Revision as of 13:49, 15 October 2014

Rhamnose promoter coupled to N-genes

nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter


Characterisation

The advantage of adding the N-genes has not been proven because the final constructs containing them (BBa_K1316007 and BBa_K1316008) did not seem to be properly induced by the presence of 2,4-DNT.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]