Difference between revisions of "Part:BBa K1493002"
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| + | Promoter fusaric acid inducible | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | A fusaric acid efflux pump within ''Pseudomonas putida'' is encoded by an operon consisting of four genes. This operon is controlled by a LysR-type gene (pp1262) which is located upstream of the operon. This gene inhibits the binding of RNA polymerase to the promoter in the intergenic region between pp1262 and the operon. Fusaric acid will block this inhibition allowing activity of the operon. (See figure 1) Hence, pp1262 and the intergenic region are isolated and put into BioBrick form, effectively acting as a Fusaric Acid inducible Promoter (FAiP). | ||
| + | https://static.igem.org/mediawiki/2014/f/f3/Wageningen_UR_sensing_Faip1.jpg<br> | ||
| + | ''Figure 1. Fusaric acid efflux pump operon present in the genome of KT2440 Pseudomonas putida.'' | ||
| − | + | ===Features=== | |
| − | + | This biobrick can only be used in any microorganism that has resistance to fusaric acid. For testing and characterizing, we used ''Pseudomonas putida''. This gene contains an illegal restriction site '''PstI''' at the repressor gene, so don't use this restriction enzyme when working with this gene. <br><br>A similar functioning BioBrick can be found here: https://parts.igem.org/Part:BBa_K1493000<br> | |
| − | < | + | This biobrick with a reporter GFP can be found here: https://parts.igem.org/Part:BBa_K1493003 |
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| − | + | ===References=== | |
| − | === | + | Hu, R.-M., et al., An Inducible Fusaric Acid Tripartite Efflux Pump Contributes to the Fusaric Acid Resistance in Stenotrophomonas maltophilia. PLoS ONE, 2012. 7(12): p. e51053.<br> |
| − | < | + | Nelson, K.E., et al., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental microbiology, 2002. 4(12): p. 799-808. |
| − | + | </p> | |
Revision as of 12:20, 15 October 2014
Fusaric acid induced promoter
Promoter fusaric acid inducible
Usage and Biology
A fusaric acid efflux pump within Pseudomonas putida is encoded by an operon consisting of four genes. This operon is controlled by a LysR-type gene (pp1262) which is located upstream of the operon. This gene inhibits the binding of RNA polymerase to the promoter in the intergenic region between pp1262 and the operon. Fusaric acid will block this inhibition allowing activity of the operon. (See figure 1) Hence, pp1262 and the intergenic region are isolated and put into BioBrick form, effectively acting as a Fusaric Acid inducible Promoter (FAiP).
Figure 1. Fusaric acid efflux pump operon present in the genome of KT2440 Pseudomonas putida.
Features
This biobrick can only be used in any microorganism that has resistance to fusaric acid. For testing and characterizing, we used Pseudomonas putida. This gene contains an illegal restriction site PstI at the repressor gene, so don't use this restriction enzyme when working with this gene.
A similar functioning BioBrick can be found here: https://parts.igem.org/Part:BBa_K1493000
This biobrick with a reporter GFP can be found here: https://parts.igem.org/Part:BBa_K1493003
References
Hu, R.-M., et al., An Inducible Fusaric Acid Tripartite Efflux Pump Contributes to the Fusaric Acid Resistance in Stenotrophomonas maltophilia. PLoS ONE, 2012. 7(12): p. e51053.
Nelson, K.E., et al., Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environmental microbiology, 2002. 4(12): p. 799-808.