Difference between revisions of "Part:BBa K1462000:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1462000 short</partinfo> | <partinfo>BBa_K1462000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | 1 | + | COXVI is obtained by PCR technique from Saccharomyces cerevisiae's genome. |
| − | + | PCR system(KOD FX from Toyobo) | |
| + | contents volume/μL | ||
| + | ------------------------------ | ||
| + | KOD FX 2×Buf 25 | ||
| + | dNTPS 10 | ||
| + | H₂O 8 | ||
| + | Foward primer 2 | ||
| + | Reverse primer 2 | ||
| + | templete 1 | ||
| + | KOD FX 2 | ||
| + | ------------------------------- | ||
| + | total 50 | ||
===Source=== | ===Source=== | ||
| − | CoxVI is obtained from Saccharomyces cerevisiae's genome | + | CoxVI is obtained from Saccharomyces cerevisiae's genome by PCR techniques |
===References=== | ===References=== | ||
Revision as of 09:44, 15 October 2014
CoxVI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
COXVI is obtained by PCR technique from Saccharomyces cerevisiae's genome. PCR system(KOD FX from Toyobo) contents volume/μL
KOD FX 2×Buf 25 dNTPS 10 H₂O 8 Foward primer 2 Reverse primer 2 templete 1 KOD FX 2
total 50
Source
CoxVI is obtained from Saccharomyces cerevisiae's genome by PCR techniques