Difference between revisions of "Part:BBa K1471011:Design"

 
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===Design Notes===
 
===Design Notes===
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We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury.
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We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury. As we already know, in eukaryotes the ribosome binding site (RBS) promotes efficient and accurate translation of mRNA. Studies have shown that every eukaryotic specie has its nucleotide preferences, 
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wich means that each specie has its own Kozak sequence. We designed this BioBrick to perform the best protein expression possible in ''Arabidopsis thaliana''.
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===Source===
 
===Source===

Revision as of 01:29, 15 October 2014


RBS (Arabidopsis Thaliana).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to discuss what was the best option to enhance the expression efficiency of our proteins of interest to bio-remediate heavy metals, as mercury. As we already know, in eukaryotes the ribosome binding site (RBS) promotes efficient and accurate translation of mRNA. Studies have shown that every eukaryotic specie has its nucleotide preferences, wich means that each specie has its own Kozak sequence. We designed this BioBrick to perform the best protein expression possible in Arabidopsis thaliana.

Source

Arabidopsis thaliana genome sequence.

References