Difference between revisions of "Part:BBa K1483003"
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The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry) | The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry) | ||
| − | [[File:SspGyrBInteinTuebingen.jpg| | + | [[File:SspGyrBInteinTuebingen.jpg|900px]] |
This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25. | This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25. | ||
Revision as of 20:55, 14 October 2014
Ssp GyrB Split Intein
N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the N-terminus of the N-intein. The C-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.
The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry)
This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]