Difference between revisions of "Part:BBa K1483003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1483003 short</partinfo>
 
<partinfo>BBa_K1483003 short</partinfo>
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<p>
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N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix.
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For immobilisation, parts need to be fused to the N-terminus of the N-intein. The C-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.
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</p>
  
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N-Intein part of the ssp GyrB split intein.
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The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry)
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Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix.
+
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For immobilisation, parts need to be fused to the C-terminus of the N-intein. The N-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.
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The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH
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(was aquired by peptide synthesis and is thus not part of the registry)
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This part can also be used in order to create fusion proteins, by attaching the C-Intein to a proein instead of the matrix.
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This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25.
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Part in RFC25 standard.
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<!-- Add more about the biology of this part here

Revision as of 20:39, 14 October 2014

Ssp GyrB Split Intein

N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the N-terminus of the N-intein. The C-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.

The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (aquired by peptide synthesis and is thus not part of the registry)

This part can also be used in order to create fusion proteins, by attaching the C-Intein to a protein instead of the matrix. The part is therefore designed in RFC25.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]