Difference between revisions of "Part:BBa K1157001:Experience"

Revision as of 19:53, 14 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157001

User Reviews

UNIQ3aac64f319747410-partinfo-00000000-QINU

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iGEM Dundee 2014

We made an exact twin of this PqsR-encoding part and rather than send it to The Registry we decided to call our version BBa_K1157001 out of respect for NTU and use it for our 2014 project. We added an HA epitope tag and showed that the protein was produced in an E. coli chassis (Dundee Figure 1) when expressed from an in-house vector called pUNI-PROM. This part was used in conjunction with a pqsA promoter that we submitted as BBa_K1315001.

Dundee 2014 Figure 1 Western blot of HA-tagged PqsR

UNIQ3aac64f319747410-partinfo-00000004-QINU

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-We made an exact twin of this PqsR-encoding part and rather than send it to The Registry we decided to call our version <partinfo>BBa_K1157001</partinfo> and use it for our 2014 project. We added an HA epitope tag and showed that the protein was produced in an <i>E. coli</i> chassis (Dundee Figure 1) when expressed from an in-house vector called pUNI-PROM. This part was used in conjunction with a <i>pqsA</i> promoter that we submitted as <partinfo>BBa_K1315001</partinfo>.
+We made an exact twin of this PqsR-encoding part and rather than send it to The Registry we decided to call our version <partinfo>BBa_K1157001</partinfo> out of respect for NTU and use it for our 2014 project. We added an HA epitope tag and showed that the protein was produced in an <i>E. coli</i> chassis (Dundee Figure 1) when expressed from an in-house vector called pUNI-PROM. This part was used in conjunction with a <i>pqsA</i> promoter that we submitted as <partinfo>BBa_K1315001</partinfo>.
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