Difference between revisions of "Part:BBa K1483002:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1483002 short</partinfo> | <partinfo>BBa_K1483002 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The part is designed in RFC25 in order to ease fusion with other parts, for example immobilisation tags. | |
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===Source=== | ===Source=== | ||
| − | The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and | + | The protein encoded by this sequence naturally occurs in <i>Bacteroides fragilis</i>. This sequence was optimized for codon usage in <i>E. coli</i> K12 and obtained by <i>in-vitro</i> gene synthesis. |
===References=== | ===References=== | ||
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<p> | <p> | ||
The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/695442642?report=genbank&log$=protalign&blast_rank=1&RID=3UEFZXJE014 NCBI]. | The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/695442642?report=genbank&log$=protalign&blast_rank=1&RID=3UEFZXJE014 NCBI]. | ||
| + | </p> | ||
| + | <p> | ||
| + | A patent claiming rights on this part can be found in the [http://appft1.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=/netahtml/PTO/srchnum.html&r=1&f=G&l=50&s1=20140220553.PGNR. United States Patent & Trademark Office] | ||
</p> | </p> | ||
Latest revision as of 19:52, 14 October 2014
α-Galactosidase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 412
Design Notes
The part is designed in RFC25 in order to ease fusion with other parts, for example immobilisation tags.
Source
The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and obtained by in-vitro gene synthesis.
References
The amino acid sequence of the protein is based on data from a publication in [http://www.nature.com/nbt/journal/v25/n4/abs/nbt1298.html Nature biotechnology]
Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.
The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/695442642?report=genbank&log$=protalign&blast_rank=1&RID=3UEFZXJE014 NCBI].
A patent claiming rights on this part can be found in the [http://appft1.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=/netahtml/PTO/srchnum.html&r=1&f=G&l=50&s1=20140220553.PGNR. United States Patent & Trademark Office]