Difference between revisions of "Part:BBa K1157001:Experience"

Revision as of 19:15, 14 October 2014

This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K1157001

User Reviews

UNIQae66616575b5a88e-partinfo-00000000-QINU

••

iGEM Dundee 2013

We made an exact twin of this PqsR-encoding part and rather than send it to The Registry we decided to call our version BBa_K1157001 and use it for our 2014 project. We added an HA epitope tag and showed that the protein was produced in an E. coli chassis (Dundee Figure 1). This part was used in conjunction with a pqsA promoter that we submitted as BBa_K1315001.

UNIQae66616575b5a88e-partinfo-00000004-QINU

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-The function of this part was characterised and quantified in a new salt-responsive device. The GFP encoded by <partinfo>BBa_E0040 </partinfo> was hooked up to an OmpR-responsive promoter (<partinfo>BBa_R0083</partinfo>) via a ribosome binding site (<partinfo>BBa_B0034</partinfo>) to produce a new composite part (a reporter) (<partinfo>BBa_K1012005</partinfo>). GFP fluorescence could be detected in an <i>E. coli</i> chassis, and we used an <i>envZ</i> mutant strain to show the sensor was working correctly.
+We made an exact twin of this PqsR-encoding part and rather than send it to The Registry we decided to call our version <partinfo>BBa_K1157001</partinfo> and use it for our 2014 project. We added an HA epitope tag and showed that the protein was produced in an <i>E. coli</i> chassis (Dundee Figure 1). This part was used in conjunction with a <i>pqsA</i> promoter that we submitted as <partinfo>BBa_K1315001</partinfo>.
 |}
 ;