Difference between revisions of "Part:BBa K1381013"

 
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<partinfo>BBa_K1381013 short</partinfo>
 
<partinfo>BBa_K1381013 short</partinfo>
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<br><br><i>cheZ</i> ([https://parts.igem.org/Part:BBa_K629003 BBa_K629003]) combined with an RBS ([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]) and constitutive promoter ([https://parts.igem.org/Part:BBa_J23100 BBa_J23100]). This part was created to see if expression of <i>cheZ</i> in a <i>cheZ</i>-knockout strain would restore its motility. Similar parts are: [https://parts.igem.org/Part:BBa_K1381011 BBa_K1381011] and [https://parts.igem.org/Part:BBa_K1381012 BBa_K1381012].
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<br><br>
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<b><i>cheZ</i></b><br>
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Chemotaxis is the cell’s system for directed movement which allows the cell to move towards nutrition sources as well as away from toxic substances. The chemotactic system is a complex chain of phosphorylation reactions. At the end of the chain is the flagellum-binding protein CheY. The phosphorylated CheY (CheY-p) binds the flagella and causes the cell to tumble in place, essentially remaining immotile. The phosphatase CheZ dephosphorylates CheY, causing CheY to unbind to the flagella, resulting in directed movement of the cell [1].
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<b>References</b><br>
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[1]  <i>Madigan MT, Martinko JM, Stahl DA, Clark DP. 2010. Brock biology of microorganisms. 13th ed. Benjamin Cummings, San Francisco.</i><br>
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:22, 14 October 2014

J23100-B0034-CheZ

cheZ (BBa_K629003) combined with an RBS (BBa_B0034) and constitutive promoter (BBa_J23100). This part was created to see if expression of cheZ in a cheZ-knockout strain would restore its motility. Similar parts are: BBa_K1381011 and BBa_K1381012.

cheZ
Chemotaxis is the cell’s system for directed movement which allows the cell to move towards nutrition sources as well as away from toxic substances. The chemotactic system is a complex chain of phosphorylation reactions. At the end of the chain is the flagellum-binding protein CheY. The phosphorylated CheY (CheY-p) binds the flagella and causes the cell to tumble in place, essentially remaining immotile. The phosphatase CheZ dephosphorylates CheY, causing CheY to unbind to the flagella, resulting in directed movement of the cell [1].

References
[1] Madigan MT, Martinko JM, Stahl DA, Clark DP. 2010. Brock biology of microorganisms. 13th ed. Benjamin Cummings, San Francisco.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]