Difference between revisions of "Part:BBa K1350007"
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== Improved endoglucanase Cel5A == | == Improved endoglucanase Cel5A == | ||
BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme. | BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme. | ||
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Revision as of 17:20, 14 October 2014
Cel5A, endoglucanase（BBa_K805011 Improved）
The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.
Usage and Biology
Different from BBa_K805011 given by iGEM12_HUST-China, we have done the mutation of an ? to ?? and change the sequence in a way. However,because of the degeneracy of codon,the different sequence can encode the same enzyme.
Improved endoglucanase Cel5A
BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme.