Difference between revisions of "Part:BBa K1350006:Design"

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===Design Notes===
 
===Design Notes===
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The sequence of Mdfa was from GenBank. After primer design, pcr and gene sequencing, we linked Mdfa to a strong promoter and RBS(BBa_K608002). Then the product, Mdfa device(BBa_K1350006) has been done.
+
The sequence of Mdfa was from GenBank. After primer design, PCR and gene sequencing, we linked Mdfa to a strong promoter and RBS(BBa_K608002). Then the product, Mdfa device(BBa_K1350006) has been done.
  
 
===Source===
 
===Source===

Revision as of 16:49, 14 October 2014


promoter+RBS(BBa_K608002)+Mdfa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of Mdfa was from GenBank. After primer design, PCR and gene sequencing, we linked Mdfa to a strong promoter and RBS(BBa_K608002). Then the product, Mdfa device(BBa_K1350006) has been done.

Source

By PCR

References