Difference between revisions of "Part:BBa K1381011"
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<b><i>cheZ</i></b><br>
 
<b><i>cheZ</i></b><br>
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<i>cheZ</i> is a gene involved in the chemotactic pathway of <i>E.coli</i>. CheZ induces directed movement of the cell by dephosphorylating the flagella-binding protein CheY, causing CheY to unbind to the flagella. Thus, when <i>cheZ</i> is expressed, the cell will be motile and when <i>cheZ</i> is <i>not</i> expressed the cell will be immotile.
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Chemotaxis is the cell’s system for directed movement which allows the cell to move towards nutrition sources as well as away from toxic substances. The chemotactic system is a complex chain of phosphorylation reactions. At the end of the chain is the flagellum-binding protein CheY. The phosphorylated CheY (CheY-p) binds the flagella and causes the cell to tumble in place, essentially remaining immotile. The phosphatase CheZ dephosphorylates CheY, causing CheY to unbind to the flagella, resulting in directed movement of the cell [1].
 
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<b>References</b><br>
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[1]  <i>Madigan MT, Martinko JM, Stahl DA, Clark DP. 2010. Brock biology of microorganisms. 13th ed. Benjamin Cummings, San Francisco.</i><br>
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:47, 14 October 2014

J23113-B0034-CheZ

cheZ combined with an RBS and constitutive promoter.

cheZ
Chemotaxis is the cell’s system for directed movement which allows the cell to move towards nutrition sources as well as away from toxic substances. The chemotactic system is a complex chain of phosphorylation reactions. At the end of the chain is the flagellum-binding protein CheY. The phosphorylated CheY (CheY-p) binds the flagella and causes the cell to tumble in place, essentially remaining immotile. The phosphatase CheZ dephosphorylates CheY, causing CheY to unbind to the flagella, resulting in directed movement of the cell [1].

References
[1] Madigan MT, Martinko JM, Stahl DA, Clark DP. 2010. Brock biology of microorganisms. 13th ed. Benjamin Cummings, San Francisco.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]