Difference between revisions of "Part:BBa K1350006"
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<!-- This process is mainly activated by the cross membrane proton electrochemical gradient,
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===Usage and Biology===
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  This process is mainly activated by the cross membrane proton electrochemical gradient,
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changing the electrochemical electric potential of proton built by respiration chain to the
 
changing the electrochemical electric potential of proton built by respiration chain to the
 
  cross membrane Na+ electrochemical potential. From research about the Na+/ H+ antiporter of  
 
  cross membrane Na+ electrochemical potential. From research about the Na+/ H+ antiporter of  
 
E.coli, mdfa can pump Na+ out of cell when it pumps H+ into cell and ATP is consumed through the process.
 
E.coli, mdfa can pump Na+ out of cell when it pumps H+ into cell and ATP is consumed through the process.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:24, 14 October 2014

promoter+RBS(BBa_K608002)+Mdfa 

  The resistance of Escherichia coli to many drugs simultaneously (multidrug resistance)

often involves the expression of membrane transporters (Mdrs);MdfA,the known bacterial MDR transporters probably utilize the membrane potential(Δψ) and/or the proton gradient (ΔpH) as the driving force for drug export. 

 In Escherichia coli, Mdfa are identified to function in maintenance of a stable cytoplasmic

pH under conditions of alkaline stress.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]