Difference between revisions of "Part:BBa K1483000:Design"

(→‎Source)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
The protein encoded by this sequence naturally occurs in <i>Elizabethkingia meningoseptica</i> (syn.: <i>Chryseobacterium meningosepticum</i> and <i>Flavobacterium meningosepticum</i>). This sequence was optimized for codon usage in <i>E. coli</i> K12 and synthesized.
+
The protein encoded by this sequence naturally occurs in <i>Elizabethkingia meningoseptica</i> (syn.: <i>Chryseobacterium meningosepticum</i> and <i>Flavobacterium meningosepticum</i>). This sequence was optimized for codon usage in <i>E. coli</i> K12 and obtained by <i>in-vitro</i> gene synthesis.
  
 
===References===
 
===References===

Revision as of 14:17, 14 October 2014

α-N-Acetylgalactosamindase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 405


Design Notes

Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags. Codon usage was modified for optimal expression in E. coli K12 and common restriction sites were removed.

Source

The protein encoded by this sequence naturally occurs in Elizabethkingia meningoseptica (syn.: Chryseobacterium meningosepticum and Flavobacterium meningosepticum). This sequence was optimized for codon usage in E. coli K12 and obtained by in-vitro gene synthesis.

References

The sequence is based on data from a publication in [http://www.nature.com/nbt/journal/v25/n4/abs/nbt1298.html Nature biotechnology]

Liu, Qiyong P., et al. "Bacterial glycosidases for the production of universal red blood cells." Nature biotechnology 25.4 (2007): 454-464.

The sequence can be found in [http://www.ncbi.nlm.nih.gov/protein/205750037?report=genbank&log$=protalign&blast_rank=1&RID=3644MZXS01R NCBI].