Difference between revisions of "Part:BBa K1492000:Design"

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===Source===
 
===Source===
  
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Outer Membrane Protein X. More information see Protein Data Bank or http://www.addgene.org/23336/
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Circulairy Permutated OMPx.  
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Rice, J. J.. "Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands." Protein Science 15.4 (2006): 825-836. Print.
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http://www.addgene.org/23336/
  
 
===References===
 
===References===

Latest revision as of 10:47, 14 October 2014


Clickable Outer Membrane Protein x


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To integrate a Non-natural amino acid in the sequence of CPX some modifications were made. Within the part in which originally random mutations were induced a codon was mutated into the amber stop codon TAG. With a specific tRNA it is possible to implement a Non-Natural amino acid at the place of the TAG codon. The TAG codon was introduced into the Peptide Library by Site-Directed Mutagenesis. A HA-tag was also added to the C-terminus of the protein using overhang primers to use in characterization. After the modifications the protein was renamed to Clickable Outer Membrane Protein X (COMPx).

BBa k1492000 fig2.jpg
Figure 2 Gene design of COMPx

Source

Circulairy Permutated OMPx. Rice, J. J.. "Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands." Protein Science 15.4 (2006): 825-836. Print. http://www.addgene.org/23336/

References