Difference between revisions of "Part:BBa K1424003"

Revision as of 10:14, 14 October 2014

Kanamycin resistance for Synechocystis

The kanamycin resistance gene tn903 aph from E. coli for use with Synechocystis sp. PCC 6803.

The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006).

Usage and Biology

BBa_K1424000 [[1]] & BBa_K1424001 [[2]] were used (flanking a kanamycin resistance cassette designed for Synechocystis sp. PCC 6803; BBa_K1424003) as double homologous recombination sequences for insertion of exogenous DNA into the genome of Synechocystis sp. PCC 6803. Colonies were picked and segregated on kanamycin containing BG11 growth media. The inclusion of exogenous DNA into the host genome was verified by performing a band-shift colony PCR. Using left homologous region forward primer and right homologous region reverse primer, PCR amplification of this genomic region can be performed. The increase in band size (band shift) of the mutant strain respective of the wild type strain confirms integration of exogenous kanamycin resistance cassette into the host genome.

ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to select positive integrants in the Synechocystis sp. PCC 6803 genome.

Improved Function

This part is an improvement to the documented part BBa_K300989. It has been optimized for use in the organism Synechocystis sp. PCC 6803, and also optimized to be compatible with iGEM assembly.

Sequence and Features