Difference between revisions of "Part:BBa K1424000"

Revision as of 09:50, 14 October 2014

Prefix homologous recombination for Synechocystis ("Left flank")

Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000).

This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.

to be used with BBa_K1424001

Usage and Biology

ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 441
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1424000 short</partinfo>
+Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000).
 This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.
@@ Line 8: / Line 10: @@
 [[File:Conformation.jpg‎]]
+===Usage and Biology===
+ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function).
 <!-- Add more about the biology of this part here