Difference between revisions of "Part:BBa K1520509"

Revision as of 04:44, 14 October 2014

PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions. It can express rfp when both gold ions and IPTG are not exist. Here rfp represent lysozyme, since the plasmid with lysozyme is hard to reconstruct. When the bacteria turns red, it means they commit suicide and the following pictures shows that the system work.

From left to right: 1.add gold 2.without gold 3.add IPTG 4.without IPTG

The fluorescence intensity of each treatment

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3611
Illegal NheI site found at 3634
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4812
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 548
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1520509 short</partinfo>
@@ Line 5: / Line 4: @@
 PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
 It is a test of our suicide circuit for gold ions.
+It can express rfp when both gold ions and IPTG are not exist. Here rfp represent lysozyme, since the plasmid with lysozyme is hard to reconstruct. When the bacteria turns red, it means they commit suicide and the following pictures shows that the system work.
+[[File:goldandiptg.jpg]]
+From left to right: 1.add gold 2.without gold 3.add IPTG 4.without IPTG
+[[File:Fluo.jpg]]
+The fluorescence intensity of each treatment
 <!-- Add more about the biology of this part here