Difference between revisions of "Part:BBa K1401007"

Revision as of 00:11, 14 October 2014

Tandem promoter pTet-pBad

This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This tandem promoter is in pSB1C3.

The promoter can be induced by anhydrotetracycline and arabinose, as shown below. In order to test this tandem promoter, we built a basic transcriptional unit with red fluorescent protein reporter. We used the E. coli DH5-alpha Pro strain for this study, which has tetR, lacI, and araC all constitutively expressed in its genome.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 324
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 159
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 141

@@ Line 4: / Line 4: @@
 This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This tandem promoter is in pSB1C3.
-The promoter can be induced by anhydrotetracycline and arabinose, as shown below.
+The promoter can be induced by anhydrotetracycline and arabinose, as shown below. In order to test this tandem promoter, we built a basic transcriptional unit with red fluorescent protein reporter. We used the ''E. coli'' DH5-alpha Pro strain for this study, which has ''tetR'', ''lacI'', and ''araC'' all constitutively expressed in its genome.
 [[File:K1401007_flow.png|center|900px]]