Difference between revisions of "Part:BBa K1350007"

(Usage and Biology)
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<partinfo>BBa_K1350007 short</partinfo>
 
<partinfo>BBa_K1350007 short</partinfo>
  
we got this endoglucanase from iGEM kit,and we mutate it in a certain spot.[https://parts.igem.org/Part:BBa_K805011]<br>
 
 
===Cel5A, endoglucanases===
 
===Cel5A, endoglucanases===
 
The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.
 
The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Revision as of 15:56, 13 October 2014

Cel5A, endoglucanase(BBa_K805011 Improved)

Cel5A, endoglucanases

The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Usage and Biology

Different from BBa_K805011 given by iGEM12_HUST-China, we have done the mutation of an ? to ?? and change the sequence in a way. However,because of the degeneracy of codon,the different sequence can encode the same enzyme.