Difference between revisions of "Part:BBa K1468000"

Revision as of 15:10, 13 October 2014

pJ23104 + gene encoding ZsGreen

Contents

Description
Usage and Biology
Universality
Compatibility with current standards and systems (Modularity)
Innovative point
FAQ
Further reading and additional information

Description

This part consists of the ZsGreen coding sequence of Zoanthus spp., placed under the control of the constitutive promoter J23104 -promoter designed by J. Anderson (iGEM 2006, Berkeley). This promoter belongs to a family of constitutive promoter parts isolated from a small combinatorial library.

Usage and Biology

The biobrick promoter J23104, as it was mentioned above, is constitutive. It was tested by two groups of iGEMites, additonally to Anderson's: 2012 iGEM Team Göttingen (by C. Krüger and J. Kampf) and iGEM Cinestav (National Autonomous University of Mexico & National Polytechnic Institute; Center for Research and Advanced Studies at Irapuato).

Results obtained here: [1]

Universality

A priori, BBa_K1468000 can be used in a wide variety of prokariotic and eukariotic cells.

However, some considerations should be taken into account:

- The J23104 promoter, constitutive, has been tested just in Escherichia coli strains. This fact ensures its correct operation in a group of closed related species but not to the wide range of microorganism.

- The ZsGreen encoding gene sequence is optimized forEscherichia coli codon usage. Some codon usage modification must be carried out for non-related organisms.

- There are some limitations related to the plasmid, where the construction is cloned. I mean, the backbones Ori is not universal, so it would be necessary to check that if Ori is compatible with the organism we want to transform.

Part Stability

Compatibility with current standards and systems (Modularity)

Innovative point

FAQ

Further reading and additional information

Sequence and Features