Difference between revisions of "Part:BBa K1350003:Design"

 
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<partinfo>BBa_K1350003 short</partinfo>
 
<partinfo>BBa_K1350003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The original sequence consists of 2412 bp and we pcr to get only 1905bp of them because we don't want our target gene has xbaI Restriction Enzyme cutting site.As expected,the function of this enzyme is not changed at all.
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The original sequence consists of 2412 bp and we pcr to get only 2087bp of them because we don't want our target gene has xbaI Restriction Enzyme cutting site.As expected,the function of this enzyme is not changed at all.
  
  

Revision as of 14:06, 13 October 2014

Alkaline Cellulase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence consists of 2412 bp and we pcr to get only 2087bp of them because we don't want our target gene has xbaI Restriction Enzyme cutting site.As expected,the function of this enzyme is not changed at all.


Source

bacillus subtilis

References