Difference between revisions of "Part:BBa K1463000"
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A recombinase switch with GFP one side of the switch and RFP on the other. | A recombinase switch with GFP one side of the switch and RFP on the other. | ||
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| + | [[Image:GU_jake_gel_1.jpg|thumb|500px|right|'''Fig. 1:''' 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.]] | ||
https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg | https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg | ||
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Revision as of 12:51, 13 October 2014
Recombinase Switch With GFP and RFP
A recombinase switch with GFP one side of the switch and RFP on the other.
Fig. 1: 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 813
Illegal NheI site found at 836 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 793
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 34
Illegal AgeI site found at 146 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 711
Illegal BsaI.rc site found at 1668