Difference between revisions of "Part:BBa K1463000"
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A recombinase switch with GFP one side of the switch and RFP on the other. | A recombinase switch with GFP one side of the switch and RFP on the other. | ||
| − | + | [[Image:GU_jake_gel_1.jpg|thumb|500px|right|'''Fig. 1:''' BL21 ''E. coli'' carrying different constructs on [http://2012.igem.org/Team:Goettingen/Project/Materials#M9_Swimming_Agar M9 minimal medium swarming agar]. after 48h incubation at 33°C. Cells expressing ''fliC'' in puc18 under the natural promoter travelled approximately 1,5cm (radius) whereas no swimming could be detected for the control plasmid carrying [https://parts.igem.org/Part:BBa_K777125 K777125]. On tryptone swimming agar ''yhjH'' transformants were usually faster.]] | |
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| − | [[Image:GU_jake_gel_1.jpg|thumb|500px|right|'''Fig. 1:''' BL21 ''E. coli'' carrying different constructs on ]] | + | |
Revision as of 12:08, 13 October 2014
Recombinase Switch With GFP and RFP
A recombinase switch with GFP one side of the switch and RFP on the other.
Fig. 1: BL21 E. coli carrying different constructs on [http://2012.igem.org/Team:Goettingen/Project/Materials#M9_Swimming_Agar M9 minimal medium swarming agar]. after 48h incubation at 33°C. Cells expressing fliC in puc18 under the natural promoter travelled approximately 1,5cm (radius) whereas no swimming could be detected for the control plasmid carrying K777125. On tryptone swimming agar yhjH transformants were usually faster.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 813
Illegal NheI site found at 836 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 793
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 34
Illegal AgeI site found at 146 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 711
Illegal BsaI.rc site found at 1668