Difference between revisions of "Part:BBa K1463000"

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A recombinase switch with GFP one side of the switch and RFP on the other.
 
A recombinase switch with GFP one side of the switch and RFP on the other.
  
 +
[[Image:motAmotB GU_jake_gel_1.jpg‎|thumb|500px|right|'''Fig. 1:''' BL21 ''E. coli'' carrying different constructs on [https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg]
  
  

Revision as of 12:00, 13 October 2014

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

[[Image:motAmotB GU_jake_gel_1.jpg‎|thumb|500px|right|Fig. 1: BL21 E. coli carrying different constructs on [1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668