Difference between revisions of "Part:BBa K1439000:Experience"
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| + | Conjugate between ''E.coil'' HB101 and ''E.coil'' Top10. | ||
Revision as of 11:46, 13 October 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1439000
We have test the oriT BBa_K1439000 in a conjugation system in which pSB1C3 with the help of plasmid RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriT BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriT BBa_K1439000. We get HB101 without streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.
User Reviews
UNIQ7b5a12ca75ef87b6-partinfo-00000000-QINU UNIQ7b5a12ca75ef87b6-partinfo-00000001-QINU Test
Conjugate between E.coil HB101 and E.coil Top10.