Difference between revisions of "Part:BBa K1442110"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1442110 short</partinfo>
 
<partinfo>BBa_K1442110 short</partinfo>
  
−
...
+
== Construction ==
 +
<p>This was the complete testing module used in the testing of the NKRF IRES, results can be seen on <html><body><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1442024">Part:BBa_K1442024</a></body></html>. This uses the empty testing GFP sequence <html><body><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1442106">Part:BBa_K1442106</a></body></html>, which has been codon optimised for human use using the <html><body><a href="https://www.idtdna.com/CodonOpt">IDT codon optimiser</a></body></html> from the original gene derived from Aequorea victoria jellyfish gene <html><body><a href="https://parts.igem.org/Part:BBa_E0040">Part:BBa_E0040</a></body></html>.</p>
 +
<p>This was measured in Huh 7.5 and HeLa cell with a tecan magellan machine.</p>
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:17, 13 October 2014

NKRF IRES with forward GFP

Construction

This was the complete testing module used in the testing of the NKRF IRES, results can be seen on Part:BBa_K1442024. This uses the empty testing GFP sequence Part:BBa_K1442106, which has been codon optimised for human use using the IDT codon optimiser from the original gene derived from Aequorea victoria jellyfish gene Part:BBa_E0040.

This was measured in Huh 7.5 and HeLa cell with a tecan magellan machine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1289
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 375
    Illegal SapI site found at 734