Difference between revisions of "Part:BBa K1381010"

 
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<partinfo>BBa_K1381010 short</partinfo>
 
<partinfo>BBa_K1381010 short</partinfo>
  
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A construct used to characterize the yenbox fused with the constitutive Anderson promoter J23101 (BBa_J23101). The yenbox_J23101 was coupled to GFP and the base level of expression togheter with the induced level of expression when double transformed with J23110-B0034-YenR (BBa_K1381005), J23102-B0034-YenR (BBa_K1381006) or J23101-B0034-YenR (BBa_K1381007) were supposed to be measured using a FACS.
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This construct was created for characterising the quorum sensing system from <i>Yersinia enterocolitica</i>, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.
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The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K1381002 BBa_K1381002] (yenbox_J23101) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).
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Too read more about the Yen system, see [https://parts.igem.org/Part:BBa_K1381004 BBa_K1381004] (B0034-YenR).
  
 
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Revision as of 20:42, 12 October 2014

yenbox_J23101-B0032-GFP

This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K1381004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.


The BioBrick that were used for assembling this construct was BBa_K1381002 (yenbox_J23101) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032). Too read more about the Yen system, see BBa_K1381004 (B0034-YenR).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
    Illegal NheI site found at 75
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751