Difference between revisions of "Part:BBa K1381008"
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This construct was created for characterising the quorum sensing system from <i>Yersinia enterocolitica</i>, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.
 
This construct was created for characterising the quorum sensing system from <i>Yersinia enterocolitica</i>, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.
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The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).
 
The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).
 
Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT).
 
Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT).
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Revision as of 20:35, 12 October 2014

yenbox_WT-B0032-GFP

This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K138004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.


The BioBrick that were used for assembling this construct was BBa_K138000 (yenbox_WT) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032). Too read more about the Yen system and for the results from the characterisation experiments, see BBa_K138004 (B0034-YenR) or BBa_K138000 (yenbox_WT).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 753