Difference between revisions of "Part:BBa K1381008"

Revision as of 20:34, 12 October 2014

yenbox_WT-B0032-GFP

This construct was created for characterising the quorum sensing system from Yersinia enterocolitica, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator BBa_K138004 (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.

The BioBrick that were used for assembling this construct was BBa_K138000 (yenbox_WT) and BBa_E0040 (the green fluorescence protein GFP with the RBS B0032). Too read more about the Yen system and for the results from the characterisation experiments, see BBa_K138004 (B0034-YenR) or BBa_K138000 (yenbox_WT).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 41
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 753

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 <partinfo>BBa_K1381008 short</partinfo>
-A construct used to characterize the yenbox fused with a wildtype promoter (BBa_K1381000) orginated from Yersinia enterocolitica. The yenbox_WT was coupled to GFP and the base level of expression togheter with the induced level of expression when double transformed with J23110-B0034-YenR (BBa_K1381005), J23102-B0034-YenR (BBa_K1381006) or J23101-B0034-YenR (BBa_K1381007) was measured using a FACS.
+This construct was created for characterising the quorum sensing system from <i>Yersinia enterocolitica</i>, the Yen system. yenbox_WT is one of the parts in the Yen system, a recognition region (homologous to the luxbox from the well known Lux system), fused with a wildtype promoter. When interaction between the activator [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) and the yenbox occur, the expression level of the fused promoter is induced.
+The BioBrick that were used for assembling this construct was [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040] (the green fluorescence protein GFP with the RBS B0032).
+Too read more about the Yen system and for the results from the characterisation experiments, see [https://parts.igem.org/Part:BBa_K138004 BBa_K138004] (B0034-YenR) or [https://parts.igem.org/Part:BBa_K138000 BBa_K138000] (yenbox_WT).
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