Difference between revisions of "Part:BBa K1381018"

Revision as of 20:24, 12 October 2014

B0034-USP45-(GS)x5

USP45 USP45 is en export tag that works in lactobacillus as well as E.coli.

The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part contains the RBS and export tag USP45. In team Uppsala iGEM 2014 project this part was to be coupled with the colicin Fy BBa_K1381017. The biobrick suffix is RFC25 and contains an AgeI restriction site for easier fusion with other protein sequences (The Colicin Fy BBa_K1381017 part was designed with an RFC25 prefix with NgoMIV site).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 130
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:53, 12 October 2014 (view source) Amaron (Talk \| contribs) ← Older edit		Revision as of 20:24, 12 October 2014 (view source) Vktorision (Talk \| contribs) Newer edit →
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	'''USP45''' USP45 is en export tag that works in ''lactobacillus'' as well as ''E.coli.''		'''USP45''' USP45 is en export tag that works in ''lactobacillus'' as well as ''E.coli.''

−	'''The killing system''' We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part contains the RBS and export tag USP45. In team Uppsala iGEM 2014 project this part was to be ~~copled~~ with the colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017]. It contains an AgeI restriction site for ~~a more easily~~ fusion with other protein ~~sequence.~~(The Colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017] part was designed with ~~a MgoMIV~~ site) ~~It does not follow the Freiburg Biobrick standard 25 since it does not have an MgoMIV site~~.	+	'''The killing system''' We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part contains the RBS and export tag USP45. In team Uppsala iGEM 2014 project this part was to be coupled with the colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017]. The biobrick suffix is RFC25 and contains an AgeI restriction site for easier fusion with other protein sequences (The Colicin Fy [https://parts.igem.org/Part:BBa_K1381017 BBa_K1381017] part was designed with an RFC25 prefix with NgoMIV site).