Difference between revisions of "Part:BBa K1420001"

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catalyze the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. </p>
 
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''merA'' is located downstream of merR (encodes for Mer Operon regulatory Mer Repressor) and two genes encode. for mercuric ion transport protein, MerB and MerT.
 
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<p>While orchestrating with the mercury transport system,  MerA and <a href="https://parts.igem.org/Part:BBa_K1420002">MerB</a> are the key detoxification  enzymes that confer mercury resistance.</p>
 
  
 
== Function ==
 
== Function ==
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MerA catalyzes the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. </p>
  
 
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''Scheme 1.'' MerA Catalyzed Reaction
 
''Scheme 1.'' MerA Catalyzed Reaction
 
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[[File:MerA_reaction Mechanism]]
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[[File:merA_Reaction_mechanism.jpg]]
 
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Revision as of 19:55, 12 October 2014

merA, mercuric reductase from Serratia marcescens

Overview

Mercury resistant gene merA encodes a mercuric ion reductase, MerA, that couples with organomercurial lyase, MerB, to detoxify inorganic and organic mercury compound. While MerA and MerB are the key enzymes of mercury detoxification, they orchestrate with mercury transport proteins, MerT and MerP, to confer bacterial mercury resistance. Figure 1 shows the interactions of MerA and other proteins encoded by Mer Operon.

File:MerA in Mer Operon.jpg


Function

MerA catalyzes the reduction of mercuric ion(Hg2+) to the relative inert, volatile monoatomic mercury (Hg0) in a NADPH dependent reaction. </p>

Scheme 1. MerA Catalyzed Reaction

MerA Reaction mechanism.jpg 

 </p>

Structure and Mechanism

Zone of Inhibition Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1249
    Illegal NgoMIV site found at 1311
    Illegal NgoMIV site found at 1522
  • 1000
    COMPATIBLE WITH RFC[1000]