Difference between revisions of "Part:BBa K1396001"

Latest revision as of 19:49, 12 October 2014

pSB1C3: LL-37_MFP

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action.

This is an improvement on the Utah State biobrick BBa_K1162006 which consists of only the LL-37 peptide.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 232
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1396001 short</partinfo>
-he part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.
+The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine suppressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence suppressing LL-37 antimicrobial action.
+This is an improvement on the Utah State biobrick  [https://parts.igem.org/Part:BBa_K1162006 BBa_K1162006] which consists of only the LL-37 peptide.
 <!-- Add more about the biology of this part here