Difference between revisions of "Part:BBa K1420001"
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<p>Mercury resistant gene''merA'' encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of ''Mer'' Operon (MerT, MerR, MerP), MerA and [MerB] are the key detoxification  enzymes that confer mercury resistance.  
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<p>Mercury resistant gene''merA'' encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of ''Mer'' Operon (MerT, MerR, MerP), MerA and <a href="https://parts.igem.org/Part:BBa_K1420002">MerB</a> are the key detoxification  enzymes that confer mercury resistance. </p>
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''Scheme 1.'' MerA Catalyzed Reaction
 
''Scheme 1.'' MerA Catalyzed Reaction

Revision as of 19:17, 12 October 2014

merA, mercuric reductase from Serratia marcescens

Function

Mercury resistant genemerA encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg2+) to the relative inert, volatile monoatomic mercury (Hg0) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of Mer Operon (MerT, MerR, MerP), MerA and <a href="https://parts.igem.org/Part:BBa_K1420002">MerB</a> are the key detoxification enzymes that confer mercury resistance.

Scheme 1. MerA Catalyzed Reaction

File:MerA reaction Mechanism 

 </p>

Structure and Mechanism

Zone of Inhibition Results

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1249
    Illegal NgoMIV site found at 1311
    Illegal NgoMIV site found at 1522
  • 1000
    COMPATIBLE WITH RFC[1000]