Difference between revisions of "Part:BBa K1420001"

Revision as of 19:08, 12 October 2014

merA, mercuric reductase from Serratia marcescens

Function

Mercury resistant genemerA encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg²⁺) to the relative inert, volatile monoatomic mercury (Hg⁰) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of Mer Operon (MerT, MerR, MerP), MerA and [MerB] are the key detoxification enzymes that confer mercury resistance. Scheme 1. MerA Catalyzed Reaction <p>

File:MerA reaction Mechanism

 </p>

Structure and Mechanism

Zone of Inhibition Results

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1201
Illegal NgoMIV site found at 1249
Illegal NgoMIV site found at 1311
Illegal NgoMIV site found at 1522
1000
COMPATIBLE WITH RFC[1000]

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-<p>Mercury resistant gene''merA'' encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of ''Mer'' Operon, MerA and [[MerB]] are the key detoxification  enzymes that confer mercury resistance.
+<p>Mercury resistant gene''merA'' encodes a a mercuric ion reductase (MerA) that catalyze the reduction of mercuric ion(Hg<sup>2+</sup>) to the relative inert, volatile monoatomic mercury (Hg<sup>0</sup>) in a NADPH dependent reaction. While orchestrating with the transportation system composed of other gene products of ''Mer'' Operon (MerT, MerR, MerP), MerA and [MerB] are the key detoxification  enzymes that confer mercury resistance.
 ''Scheme 1.'' MerA Catalyzed Reaction