Difference between revisions of "Part:BBa K1520507"
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A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions. | A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions. | ||
| + | GolB is a identified small gold-specific protein in S. typhimurium that is highly induced in the presence of Au(I) over Cu(I), Ag(I), Zn(II), or Hg(II) ions. It has less than 70 amino acids in length and contains a similar metal binding motif, MTCXXC, to that of the previously reported human copper chaperone AtoX1 and yeast copper chaperone AtX1. | ||
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| + | We display GolB on E. coli cell surface by fusing it with OmpA, an E. coli outer membrane protein to increase the disposing efficiency. The Lpp–OmpA fusion system has been previously used to display proteins as large as 263 amino acids on the surface of E. coli. Following a similar protocol, we constructed a plasmid carrying the OmpA–GolB expression gene by connecting the N-terminal 1–159 amino acids of OmpA with the full-length GolB (Fig. 1a) | ||
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| + | [[File:golB.jpg]] | ||
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Revision as of 18:25, 12 October 2014
OMPA-golB
OMPA-golB A protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions.
GolB is a identified small gold-specific protein in S. typhimurium that is highly induced in the presence of Au(I) over Cu(I), Ag(I), Zn(II), or Hg(II) ions. It has less than 70 amino acids in length and contains a similar metal binding motif, MTCXXC, to that of the previously reported human copper chaperone AtoX1 and yeast copper chaperone AtX1.
We display GolB on E. coli cell surface by fusing it with OmpA, an E. coli outer membrane protein to increase the disposing efficiency. The Lpp–OmpA fusion system has been previously used to display proteins as large as 263 amino acids on the surface of E. coli. Following a similar protocol, we constructed a plasmid carrying the OmpA–GolB expression gene by connecting the N-terminal 1–159 amino acids of OmpA with the full-length GolB (Fig. 1a)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 770
Illegal XhoI site found at 742 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]