Difference between revisions of "Part:BBa K1321337"

m
Line 8: Line 8:
 
Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.  
 
Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.  
  
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K1321337 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1321337 SequenceAndFeatures</partinfo>
  

Revision as of 15:50, 12 October 2014

sfGFP in Freiburg format (RFC 25)

sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.

Usage and Biology

Superfolder Green Fluorescent Protein (sfGFP) is a mutant of mut3b GFP (E0040) with improved in vivo properties. It was first entered into the registry by the Cambridge iGEM team in 2008; please see I746916 part description and http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP for detailed information about the properties of sfGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10