Difference between revisions of "Part:BBa K1381017"

Revision as of 14:20, 12 October 2014

CFY-X6 his

Colicin Fy The CFY is a bacteriocin in the colicin family. It is produced naturally by Y.frederiksenii and target closed relatives to that bacteria. One among them is the Y.enterocolitica and our target pathogene. The Colicin Fy will target the cellmembrane of it's target.

The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1381017 short</partinfo>
-- Insert description -
+'''Colicin Fy'''
+The CFY is a bacteriocin in the colicin family. It is produced naturally by ''Y.frederiksenii'' and target closed relatives to that bacteria. One among them is the ''Y.enterocolitica'' and our target pathogene. The Colicin Fy will target the cellmembrane of it's target.
+'''The killing system'''
+We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice.
 <!-- Add more about the biology of this part here