Difference between revisions of "Part:BBa K1403003"
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<partinfo>BBa_K1403003 short</partinfo>
 
<partinfo>BBa_K1403003 short</partinfo>
  
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This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library.  
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This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:38, 12 October 2014

Limonene synthase (LIMS1) expression cassette

This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Verification

Limonene.png

Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator