Difference between revisions of "Part:BBa K1442102:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1442102 short</partinfo>
 
<partinfo>BBa_K1442102 short</partinfo>
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===Design Notes===
 
===Design Notes===
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...
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There are two restriction sites surrounding the 3’UTR  AflII and BsiW1 which can be used to change the RDRP promotor.  
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The GFP has been codon optimised for E.coli  IDTs codon optimising software
  
  
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===Source===
 
===Source===
  
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...
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The sequence for GFP was obtained from the NCBI database and was codon optimised.
  
 
===References===
 
===References===
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https://www.idtdna.com/CodonOpt

Revision as of 13:34, 12 October 2014

Reverse GFP for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 763


Design Notes

There are two restriction sites surrounding the 3’UTR AflII and BsiW1 which can be used to change the RDRP promotor.

The GFP has been codon optimised for E.coli IDTs codon optimising software


Source

The sequence for GFP was obtained from the NCBI database and was codon optimised.

References

https://www.idtdna.com/CodonOpt