Difference between revisions of "Part:BBa K1442102:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1442102 short</partinfo> | <partinfo>BBa_K1442102 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | There are two restriction sites surrounding the 3’UTR AflII and BsiW1 which can be used to change the RDRP promotor. | ||
+ | |||
+ | The GFP has been codon optimised for E.coli IDTs codon optimising software | ||
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===Source=== | ===Source=== | ||
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+ | The sequence for GFP was obtained from the NCBI database and was codon optimised. | ||
===References=== | ===References=== | ||
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+ | https://www.idtdna.com/CodonOpt |
Revision as of 13:34, 12 October 2014
Reverse GFP for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 799 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 763
Design Notes
There are two restriction sites surrounding the 3’UTR AflII and BsiW1 which can be used to change the RDRP promotor.
The GFP has been codon optimised for E.coli IDTs codon optimising software
Source
The sequence for GFP was obtained from the NCBI database and was codon optimised.