Difference between revisions of "Part:BBa K1403017"
| Line 3: | Line 3: | ||
aldB CDS under constitutive promoter from Anderson's promoter library. | aldB CDS under constitutive promoter from Anderson's promoter library. | ||
| + | |||
| + | It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter. This plasmid was transformed together with Part:BBa_K1403019 to produce butter smell in E.Coli. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:10, 12 October 2014
Acetaldehyde dehydrogenase (aldB) expression cassette
aldB CDS under constitutive promoter from Anderson's promoter library.
It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter. This plasmid was transformed together with Part:BBa_K1403019 to produce butter smell in E.Coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]
Verification
Expeceted bands at 2kbp for pSB1C3 and 846bp for aldB gene with promoter