Difference between revisions of "Part:BBa K1368008:Design"

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===Design Notes===
 
===Design Notes===
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to be finished
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Firstly, we divided this circuit into two parts: riboswitch and Cre protein with double terminators. When it came to the synthesis of riboswitch, we designed four specific primers which had almost 20 overlapping sequence and conducted twice PCR to get our riboswitch (more details are shown in our protocol). Then, we connected both of them by the standard method of biobrick connection.
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===Source===
 
===Source===

Latest revision as of 06:40, 12 October 2014


mcherry generator driven by HSL under the post transcriptional control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61
    Illegal BsaI.rc site found at 300


Design Notes

Firstly, we divided this circuit into two parts: riboswitch and Cre protein with double terminators. When it came to the synthesis of riboswitch, we designed four specific primers which had almost 20 overlapping sequence and conducted twice PCR to get our riboswitch (more details are shown in our protocol). Then, we connected both of them by the standard method of biobrick connection.

Source

All of the basic parts come from iGEM distribution.

References