Difference between revisions of "Part:BBa K1368008"

Latest revision as of 06:37, 12 October 2014

mcherry generator driven by HSL under the post transcriptional control

The right luxr promoter gives relatively weak constitutive expression of downstream genes. If the complex of LuxR in concert with HSL binds to a palindromic site on the right luxr promoter, the rate of transcription will increase. After the right luxr promoter being activated by the complex of LuxR in concert with HSL, the transcription of taR12 key will be increased. crR12 is biobricked version of Isaacs' riboregulator cis repressed lock. Furthermore, Riboregulator Lock 1 is also under the control of the right luxr promoter. The part crR12 is transcribed into mRNA and it forms a stem-loop structure which "locks" the ribosome binding site. The corresponding taR12 key will interject crR12 itself and open the loop to express the downstream mCherry-LVA which contains the fluorescent protein mCherry attached to a degradation tag. This composite part can realize the multistage regulation and allow for the strong control of the expression of downstream gene driven by HSL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 61
Illegal BsaI.rc site found at 300

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1368008 short</partinfo>
-This device is designed to test the effect of riboregulator in our input module.
+The right luxr promoter gives relatively weak constitutive expression of downstream genes.  If the complex of LuxR in concert with HSL binds to a palindromic site on the right luxr promoter, the rate of transcription will increase. After the right luxr promoter being activated by the complex of LuxR in concert with HSL, the transcription of taR12 key will be increased. crR12 is biobricked version of Isaacs' riboregulator cis repressed lock. Furthermore, Riboregulator Lock 1 is also under the control of the right luxr promoter. The part crR12 is transcribed into mRNA and it forms a stem-loop structure which "locks" the ribosome binding site. The corresponding taR12 key will interject crR12 itself and open the loop to express the downstream mCherry-LVA which contains the fluorescent protein mCherry attached to a degradation tag. This composite part can realize the multistage regulation and allow for the strong control of the expression of downstream gene driven by HSL.
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