Difference between revisions of "Part:BBa K1537028"
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<partinfo>BBa_K1537028 short</partinfo>
 
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AHA2 belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport.We want to enlarge the stomata of tobacco by overexpression of AHA2.
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Stomata are microscopic pores surrounded by two guard cells and play an important role in the uptake of CO2 for photosynthesis. Recent researches revealed that light-induced stomatal  opening is mediated by at least three key components: blue light receptor phototropin, plasma membrane H+-ATPase, and plasma membrane inward-rectifying K+ channels.However, Yin Wang, et al[1]showed that only increasing the amount of H+-ATPase in guard cells had a significant effect on light-induced stomatal opening. Transgenic Arabidopsis plants by overexpressing H+-ATPase in guard cells exhibited enhanced photosynthesis activity and plant growth.
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Therefore,in order to improve the ability of absorbing formaldehyde, we overexpresse H+-ATPase(At AHA2) in transgenic tobacco guard cells ,resulting in a significant effect on light-induced stomatal opening(Yin Wanga et al,2014).
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Fig. 1
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(A) Typical fluorescence images of immunohistochemical detection of the guard cell H+-ATPase in the Arabidopsis epidermis. (B) qPCR analysis of AHA2 expression. Error bars represent the SEM (n ≥ 6). Significant differences were detected by Student t test (***P < 0.001).
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(C) Typical stomata in the epidermis illuminated with light for 30 min.
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References
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Yin Wanga,Ko Noguchib, Natsuko Onoa, Shin-ichiro Inouea, Ichiro Terashimab, and Toshinori Kinoshitaa,(2014)Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. PNAS .111(1) .533–538
  
 
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Revision as of 03:17, 12 October 2014

AHA2

Stomata are microscopic pores surrounded by two guard cells and play an important role in the uptake of CO2 for photosynthesis. Recent researches revealed that light-induced stomatal opening is mediated by at least three key components: blue light receptor phototropin, plasma membrane H+-ATPase, and plasma membrane inward-rectifying K+ channels.However, Yin Wang, et al[1]showed that only increasing the amount of H+-ATPase in guard cells had a significant effect on light-induced stomatal opening. Transgenic Arabidopsis plants by overexpressing H+-ATPase in guard cells exhibited enhanced photosynthesis activity and plant growth. Therefore,in order to improve the ability of absorbing formaldehyde, we overexpresse H+-ATPase(At AHA2) in transgenic tobacco guard cells ,resulting in a significant effect on light-induced stomatal opening(Yin Wanga et al,2014).

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Fig. 1 (A) Typical fluorescence images of immunohistochemical detection of the guard cell H+-ATPase in the Arabidopsis epidermis. (B) qPCR analysis of AHA2 expression. Error bars represent the SEM (n ≥ 6). Significant differences were detected by Student t test (***P < 0.001). (C) Typical stomata in the epidermis illuminated with light for 30 min. References Yin Wanga,Ko Noguchib, Natsuko Onoa, Shin-ichiro Inouea, Ichiro Terashimab, and Toshinori Kinoshitaa,(2014)Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. PNAS .111(1) .533–538

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]