Difference between revisions of "Part:BBa K1355002"
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In the presence of mercury, MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and bio detection starts! MerP and MerT proteins are responsible for the transport of mercury from the periplasm to cytoplasm and GFP will act as a reporter alerting the presence of mercury in the middle and higher is mercury’s concentration, higher will be GFP expression. And if there isn’t mercury on medium, MerT, MerP and GFP synthesis will be repressed by MerR repressor protein. | In the presence of mercury, MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and bio detection starts! MerP and MerT proteins are responsible for the transport of mercury from the periplasm to cytoplasm and GFP will act as a reporter alerting the presence of mercury in the middle and higher is mercury’s concentration, higher will be GFP expression. And if there isn’t mercury on medium, MerT, MerP and GFP synthesis will be repressed by MerR repressor protein. | ||
| + | [[File:biosensor.jpg]] | ||
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Revision as of 21:32, 11 October 2014
Mercury ions detector device
We used the biobrick (BBa_E0840) available on iGEM database since 2004 to connect to our “essential bio brick” (BBa_K1355001) and - together - form a system capable of bio detection mercury in bacteria, represented below:
In the presence of mercury, MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and bio detection starts! MerP and MerT proteins are responsible for the transport of mercury from the periplasm to cytoplasm and GFP will act as a reporter alerting the presence of mercury in the middle and higher is mercury’s concentration, higher will be GFP expression. And if there isn’t mercury on medium, MerT, MerP and GFP synthesis will be repressed by MerR repressor protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 988
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 586
Illegal NgoMIV site found at 1160 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1862
Illegal SapI site found at 579