Difference between revisions of "Part:BBa K1385000"
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<partinfo>BBa_K1385000 short</partinfo> | <partinfo>BBa_K1385000 short</partinfo> | ||
| − | + | This part incorporates the CpcG2 promoter from Jeff Tabor's 2010 paper "Multichromatic Control of Gene Expression in Escherichia coli" and makes it an inverter. | |
| + | This light regulated promoter expresses TetR in the presence of light. This part should be used in conjunction with BBa_K1017726 to activate the light regulated plasmid, and a reporter plasmid such as https://parts.igem.org/Part:BBa_J70311 which is a pTet promoter a reporter protein EYFP | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:59, 11 October 2014
CPCG2 promoter -> TetR
This part incorporates the CpcG2 promoter from Jeff Tabor's 2010 paper "Multichromatic Control of Gene Expression in Escherichia coli" and makes it an inverter. This light regulated promoter expresses TetR in the presence of light. This part should be used in conjunction with BBa_K1017726 to activate the light regulated plasmid, and a reporter plasmid such as https://parts.igem.org/Part:BBa_J70311 which is a pTet promoter a reporter protein EYFP
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 279
Illegal NheI site found at 302 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]